Relationship between Microbial Degradability and Polarographic Half-Wave Potential of Poly-chlorocyclohexenes and BHC Isomers
Year: 1981
Type: article
Abstract: Partially purified, cell-free extracts of Clostridium rectum effectively degraded lindane (y-BHC) and related polychlorocyclohexenes, in the presence of dithiothreitol.The first step of the reaction was proved to be reductive dechlorination.These compoundswere also reductively dechlorinated by polarography.The half-wave potentials had a linear relationship to the logarithmic values of the biodegradation rates.In the biodegradation of toxic chlorinated hydrocarbons, C-Cl bond cleaving reactions, such as reductive dechlorination, dehydrochlorination and glutathione conjugation (substitution by glutathione), are usually critical steps in waste-scavenging metabolism and in the detoxication processes.Reductive dechlorination is the major route of lindane (y-BHC) degradation in the strict anaerobic Clostridium spp.1>2) and in the facultative anaerobic Citrobacter freundii.3)A washed cell suspension and cell-free extracts of Clostridium rectum reductively attack lindane, dehalogenate it, and produce tetrachlorocyclohexene and monochlorobenzene.4)Reductive dechlorination of lindane also is conducted by Pseudomonas putida5) and by rat liver microsomal cytochrome P-450 under anaerobic condition.6)Here we report the metabolic reactivities of a-BHC and lindane, and of various polychlorocyclohexenes in the presence of 1,4dithiothreitol (DTT**) in a partially purified fraction of the cell-free extracts obtained from C. rectum.Since these metabolic reactions * Studies on BHCIsomers and Related Compounds.XXVIII.
Institution: Kyoto University
Language: English
Subfield: Pollution
Field: Environmental Science
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W779594608