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Quantitative analysis of the erythrocyte membrane proteins in polycythemia vera patients treated with hydroxycarbamide

Year: 2015
Type: article
Study design: Observational Study
Abstract: More than 90% of polycythemia vera (PV) patients have a mutation in the protein JAK2, which is closely associated with the erythrocyte membrane. With the comparison of 1-D gels of erythrocyte membranes obtained from PV patients treated with hydroxycarbamide and those of untreated controls we observed significant differences in the region of 40–55 kDa. On the basis of the LC–MS/MS analysis of this region we report up-regulation of four protein disulfide isomerases, which was subsequently confirmed by targeted mass spectrometric analysis. In further studies it will be prudent to compare this in patients both treated and not treated with hydroxycarbamide.
DOI: 10.1016/j.euprot.2015.04.001
Authors: Darshana Kottahachchi, Lallindra Gooneratne, Anil Jayasekera, Dorota Muth-Pawlak, Robert Moulder, Susumu Y. Imanishi, Ari Ariyaratne, Anne Rokka, Garry L. Corthals
Institutions: General Sir John Kotelawala Defence University, University of Colombo, Åbo Akademi University, University of Turku, Turku Centre for Biotechnology, Meijo University, University of Amsterdam
Language: English
Subfield: Hematology
Field: Medicine
Open Access status: hybrid
Estimated APC paid: $1,700
OpenAlex ID: W795166994
FWCI: 0.221
Cites: 52
Cited by: 5
Related to: 9