Acción biorreguladora de los brasinoesteroides DAA-6. DI-31 y ME sobre la collogénesis en variedades comerciales de arroz (Oryza sativa L.)
Year: 2000
Type: article
Abstract: The effect of brassinosteroids analogous DAA-6, DI-31 and ME was evaluated looking for more efficient callus induction media, as an initial phase to rice In vitro culture establishment on mature seeds of Jucarito 104 (J-104) and Pokkali (Pok) rice commercial varieties. Seeds were dehusked and disinfected before culturing in 4 culture media. Media were combinations of Murashige and Skoog salts, 1962 (MS), hormonal supplement of 2,4 dichlorophenoxyacetic acid (2,4-D) 2,0 mg/L, Kinetin (KIN) 1,0 mg/L and the corresponding brassinosteroids analogous (BRs), at 10-5 mg/L, finally, thirteen treatments of media were tested for both varieties. The better induction response was obtained after one month of culture, with combination: MS+ 2,4-D 2,0 mg/L + BRs 1 o-s mg/L for all brassinosteroids tested. The addition of 2,4-D is corroborated as an essential requirement ori callus induction. Brassinosteroids DAA-6 was an effective substitute of KIN 1,0 mg/L when used at a concentration of 10-ll mg/L for both varieties, whereas ME had such effect only for J-104. Citation: Prede Rodríguez, M. L., Rodríguez Soria, J. B., Rodríguez Machado, L. & Coronado Hernández, Y. 2000. Acción biorreguladora de· los brasinoesteroides DAA-6, DI-31 y ME sobre la callogénesis en variedades comerciales de arroz (Oryza sativa L.). Revista Jard. Bot. Nac. Univ. Habana 21(1): 83-87. Published: 4 abril de 2000.
Institution: University of Havana
Language: Spanish
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W855942399