Open-top selective plane illumination microscope for conventionally mounted specimens
Year: 2015
Type: article
Abstract: We have developed a new open-top selective plane illumination microscope (SPIM) compatible with microfluidic devices, multi-well plates, and other sample formats used in conventional inverted microscopy. Its key element is a water prism that compensates for the aberrations introduced when imaging at 45 degrees through a coverglass. We have demonstrated its unique high-content imaging capability by recording Drosophila embryo development in environmentally-controlled microfluidic channels and imaging zebrafish embryos in 96-well plates. We have also shown the imaging of C. elegans and moving Drosophila larvae on coverslips.
Source: Optics Express
DOI: 10.1364/oe.23.016142
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,270
Funders: National Science Foundation, National Institutes of Health, National Institute of Mental Health, National Institute on Drug Abuse, National Institute of General Medical Sciences, National Institute of Neurological Disorders and Stroke, National Institute of Arthritis and Musculoskeletal and Skin Diseases
Awards: Advancing Whole-Organism Screening for Chemical Modifiers of the Brain, Microfluidic immunoprofiling for biomarker discovery in rheumatoid arthritis, Developing tools for understanding neuromodulation of hypothalamic func, UCSF/UCB Joint Graduate Group in Bioengineering, The Role of Dscam Dock and Cdc42 Effectors in Prosophilia Neuron Dendritogenesis, Caenorhabditis Genetics Center
OpenAlex ID: W862235781