Work

A method to analyze, sort, and retain viability of obligate anaerobic microorganisms from complex microbial communities

Year: 2015

Type: article

Abstract: A high speed flow cytometric cell sorter was modified to maintain a controlled anaerobic environment. This technology enabled coupling of the precise high-throughput analytical and cell separation capabilities of flow cytometry to the assessment of cell viability of evolved lineages of obligate anaerobic organisms from cocultures.

Source: Journal of Microbiological Methods

DOI: 10.1016/j.mimet.2015.07.009

Authors: Anne W. Thompson, Matthew J. Crow, Brian Wadey, Christina Arens, Serdar Turkarslan, Sergey Stolyar, Nicholas Elliott, Timothy W. Petersen, Ger van den Engh, David A. Stahl, Nitin S. Baliga

Institutions: Institute for Systems Biology, BD Biosciences (United States), University of Washington, Lawrence Berkeley National Laboratory

Language: English

Topic: Microbial Community Ecology and Physiology

Subfield: Ecology

Field: Environmental Science

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $3,120

Funders: National Institutes of Health, National Institute of General Medical Sciences, Biological and Environmental Research

Awards: G223002938, Quantitative Systems Biology

OpenAlex ID: W884337199

FWCI: 1.069

Cites: 16

Cited by: 15

Related to: 10