Investigating The Metal Binding Affinity Of Bacterial Zinc Transporters: Zupt And Truncated Znta (znta-Pina)
Year: 2013
Type: dissertation
Abstract: Zinc is essential trace elements for all living organisms and serves as cofactor and structural cofactors for more than 300 enzymes. ZupT is one of the members of ZIP super family of transporter proteins, which were identified as iron and zinc transporter in Eukaryotes at first. However, recent evidence showed that some of members can also transport other metal ions, such as manganese or cadmium, and ZIP proteins also exist in bacteria. ZupT is responsible for zinc uptake in E. coli; however, its full metal selectivity is not yet characterized. In addition, the mechanism of transport is also unknown. Therefore, my goal is to check the affinity of ZupT with different metal ions, and try to define the metal binding site by testing different mutants.
Source: Human Biology
Author: Fei Nie
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W9280666