Dicamba growth regulator promotes genotype independent somatic embryogenesis from immature zygotic embryos of tropical maize inbred lines.
Year: 2013
Type: article
Abstract: Maize is one of the most important cereal crops in Sub-Saharan Africa and an important source of energy for humans. \nHowever, the difference in the dedifferentiation frequency of immature embryos among various genotypes indicates that callus \ninduction and genetic transformation is dependent on the genotype. This phenomenon is an impediment in the fundamental process \nof improving tropical maize germplasm especially through genetic engineering. Here, five tropical maize (Zea mays L.) genotypes, \nCML 216, CML 144, A 04, E 04 and TL 21, were evaluated for callus induction on MS medium supplemented with the growth \nregulator dicamba. Embryogenic and non embryogenic callus induction was independent of genotype when young immature embryos, \n12 days after pollination (DAP) were used for tissue culture in combination with dicamba. The optimal concentration of dicamba for \ninduction of embryogenic callus in all the genotypes was 3 mg/L, which was also the concentration at which non embryogenic callus \nformation was lowest. The frequency of embryogenic callus induction ranged from 35% to 79% among the five genotypes and \nsomatic embryos regenerated R0 shoots that produced normal R1 progenies. This regeneration method is expected to facilitate the \ndevelopment of a more efficient genotype independent Agrobacterium- mediated transformation system for tropical inbred lines.
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W973961889