Work

High-Throughput Detection of DNA Double-Strand Breaks Using Image Cytometry

Year: 2015

Type: article

Abstract: Assessment of γH2AX expression for studying DNA double-strand break formation is often performed by manual counting of foci using immunofluorescence microscopy, an approach that is laborious and subject to significant foci selection bias. Here we present a novel high-throughput method for detecting DNA double-strand breaks using automated image cytometry assessment of cell average γH2AX immunofluorescence. Our technique provides an expedient, high-throughput, objective, and cost-effective method for γH2AX analysis.

Source: BioTechniques

DOI: 10.2144/000114248

Authors: Tyler L. Fowler, Alison M. Bailey, Bryan P. Bednarz, Randall J. Kimple

Institutions: University of Wisconsin–Madison, University of Wisconsin Carbone Cancer Center

Language: English

Topic: Cell Image Analysis Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,415

Funders: National Institutes of Health, National Cancer Institute