Work

Identification and characterization of milk-clotting proteases produced by two species of mold

Year: 2014

Type: article

Abstract: Two strains of fungi were isolated and identified as Aspergillus tamarii and Penicillium pinophilum which showed good enzymatic activity on casein, 1933.33U and 1822, 21U, respectively. The search for milk clotting enzymes by fermentation at acid pH on culture medium containing whey and, after purification by molecular exclusion chromatography, affinity chromatography and SDS-PAGE helped to find two enzymes having a coagulant activity and approximately molecular weight of 35 and 30 KDa for Aspergillus tamarii and Penicillium pinophilum, respectively. The optimum pH of activity was 5.5 for both strains and the optimum temperature was 50 and 35°C for A. tamarii and P. pinophilum, respectively. Key words: Aspergillus, Penicillium, milk-clotting enzyme, extraction and purification.

Source: AFRICAN JOURNAL OF BIOTECHNOLOGY

DOI: 10.5897/ajb2013.13162

Authors: Benlounissi Acha, Mechakra-Maza Acha, J. Blum Loc, A. Marquette Christophe

Institutions: University Frères Mentouri Constantine 1, Université de Lyon, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires

Language: English

Topic: Enzyme Production and Characterization

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: diamond

OpenAlex ID: W2120062844