Work

Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy

Year: 2011

Type: conference-paper

Abstract: A real-time three dimensional (3D) fluorescence imaging system incorporating wavelength-coded and multiplexed holographic gratings is presented. Holographic gratings formed in thick Phenanthrenquinone- (PQ-) Doped Poly (methyl methacrylate) (PMMA) have narrowband spectral-spatial transmittance filtering properties to generate wavelengthspectrum selective multi-focal planes within a biological object. We demonstrate the imaging modality to obtain laserinduced fluorescent tissue structures from different depths at the excitation wavelength of 355nm.

Source: Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE

DOI: 10.1117/12.874570

Authors: Yuan Luo, Ioannis Zervantonakis, Se Baek Oh, Roger Kamm, George Barbastathis

Institution: Massachusetts Institute of Technology

Language: English

Topic: Digital Holography and Microscopy

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: green

Funders: Singapore-MIT Alliance for Research and Technology Centre, National Institutes of Health

Awards: G5751952579, G7133280175, G9000164990, G9000195714