Human Primary Lung Endothelial Cells in Culture
Year: 2012
Type: article
Abstract: Pulmonary endothelial functions are critical to maintain the low pressure of the pulmonary circulation and effective diffusion capacity of the lung. To investigate pulmonary endothelial cell biology in healthy or diseased lungs, we developed methods to harvest and culture pure populations of primary pulmonary arterial endothelial cells and microvascular endothelial cells from human lung explanted at time of transplantation or from donor lungs not used in transplantation. The purity and characteristics of cultured endothelial cells is ascertained by morphologic criteria using phase contrast and electron microscopy; phenotypic expression profile for endothelial specific proteins such as endothelial nitric oxide synthase, platelet/endothelial cell adhesion molecule, and von Willbrand factor; and endothelial function assays such as Dil-acetylated low-density lipoprotein uptake and tube formation. This detailed method provides researchers with the ability to establish cells for molecular, genetic, and biochemical investigation of human pulmonary vascular diseases.
Institutions: Cleveland Clinic, Cleveland Clinic Research
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: Asthma Inflammation Research, Pulmonary Vascular-Right Ventricular Axis Research Program, Airway redox biochemistry as a deteriminant of asthma phenotype during adolescen*, Nitric Oxide in Pulmonary Hypertension, Germline and Somatic Genetic Changes in Pulmonary Arterial Hypertension, Nitric Oxide in Pulmonary Hypertension, G9000176715, G9000268204
OpenAlex ID: W2162937290