Work

ExoT of Cytotoxic Pseudomonas aeruginosa Prevents Uptake by Corneal Epithelial Cells

Year: 2000

Type: article

Abstract: The presence of invasion-inhibitory activity that is regulated by the transcriptional activator ExsA of cytotoxic Pseudomonas aeruginosa has previously been proposed. The results of this study show that both ExoT and ExoS, known type III secreted effector proteins of P. aeruginosa that are regulated by ExsA, possess this activity. Invasion was reduced 94.4% by ExoT and 96.0% by ExoS. Invasion-inhibitory activity is not linked to ADP-ribosylation activity, at least for ExoS, since a noncatalytic mutant also inhibits uptake by an epithelial cell line (invasion was reduced 96. 0% by ExoSE381A).

Source: Infection and Immunity

DOI: 10.1128/iai.68.1.403-406.2000

Authors: Brigitte A. Cowell, David Y. Chen, Dara W. Frank, Amy J. Vallis, Suzanne M. J. Fleiszig

Institutions: University of California, Berkeley, Medical College of Wisconsin

Language: English

Topic: Bacterial biofilms and quorum sensing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Eye Institute, National Institute of Allergy and Infectious Diseases