Development of a high-throughput bead based assay system to measure HIV-1 specific immune signatures in clinical samples
Year: 2017
Type: article
Abstract: The monitoring and assessment of a broadly neutralizing antibody (bnAb) based HIV-1 vaccine require detailed measurements of HIV-1 binding antibody responses to support the detection of correlates of protection. Here we describe the development of a flexible, high-throughput microsphere based multiplex assay system that allows monitoring complex binding antibody signatures. Studying a panel of 13 HIV-1 antigens in a parallel assessment of different IgG subclasses (IgG1, IgG2 and IgG3) we demonstrate the potential of our strategy. The technical advances we describe include means to improve antigen reactivity using directed neutravidin-biotin immobilization of antigens and biotin saturation to reduce background. A particular emphasis of our study was to provide tools for the assessment of reproducibility and stability of the assay system and strategies to control for variations allowing the application in high-throughput assays, where reliability of single measurements needs to be guaranteed.
Source: Journal of Immunological Methods
Institutions: University of Zurich, University Hospital Zurich
Language: English
Topic: HIV Research and Treatment
Subfield: Virology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $2,970
Awards: Using transmission networks for assessing and guiding prevention efforts in hiv, G2924732230, G3095507042, The role of humoral immunity in HIV infection - Understanding broadly neutralizing antibody evolution, G6401315242, G7171777654, Understanding HIV-1 broadly neutralizing antibody evolution – The Swiss 4.5K Screen, G7417258655, Combining molecular epidemiology approaches and mathematical models for the spread of HIV and its coinfections, G8332789991, G8586152943
OpenAlex ID: W2952125308