Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
Year: 2019
Type: article
Abstract: Analysis of protein expression in glioma is relevant for several aspects in the study of its pathology. Numerous proteins have been described as biomarkers with applications in diagnosis, prognosis, classification, state of tumor progression, and cell differentiation state. These analyses of biomarkers are also useful to characterize tumor neurospheres (NS) generated from glioma patients and glioma models. Tumor NS provide a valuable in vitro model to assess different features of the tumor from which they are derived and can more accurately mirror glioma biology. Here we describe a detailed method to analyze biomarkers in tumor NS using immunohistochemistry (IHC) on paraffin-embedded tumor NS.
DOI: 10.3791/58931
Language: English
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: Crosstalk between glioma cells & immune cells in the tumor microenvironment: Ther, HDL-Loaded Nanoparticles for Glioma Therapeutics, Novel combined immunotherapeutic strategies for glioma: using pet dogs as a large animal spontaneous model, G5197722314, Mechanisms of glioma growth and invasion novel therapeutic strategies, G5561615526, G5932715242, G645032831, Molecular mechanisms mediated by ACVR1 G328V and H3K27M in diffuse intrinsic pontine glioma biology, Tuning Biomaterials-immune cell interactions for treatment of glioblastoma multiforme, G842143505, Interactions between the tumor cells and the neuro-immune microenvironment in mutant IDH1 gliomas: implications for therapeutics, Oligodendrocyte-focused rAAV gene therapy strategies for Canavan disease and Leukodystrophies
OpenAlex ID: W2910968288