Work

Real-time impedance-based monitoring of the growth and inhibition of osteomyelitis pathogen Staphylococcus aureus biofilms treated with novel bisphosphonate-fluoroquinolone antimicrobial conjugates

Year: 2020

Type: article

Abstract: Anterior cruciate ligament (ACL) rupture increases the risk for osteoarthritis (OA).This study aims to clarify temporal alterations in subchondral bone (SCB) and articular cartilage in a rabbit model with surgical ACL transection (ACLT).Operated (ACLT) and contralateral (C-L) knees were harvested two (n=8) and eight (n=6) weeks after ACLT, surgery together with collection of age-matched control (CNTRL, n=8) knees.Each knee was divided into lateral and medial condyles and plateaus, groove and patella.Bone morphology was quantified using micro-computed tomography.Thickness and fixed charged density (FCD) of the cartilage were measured with optical coherence tomography and digital densitometry, respectively.Trabeculae in ACLT knees were thinner compared to C-L in the lateral plateau (-6.7%;p=0.007)and groove (-10.9%;p=0.015)two weeks after surgery.At eight weeks, thinner trabeculae compared to C-L were observed at all locations.Trabecular bone volume fraction was decreased in ACLT compared to CNTRL at all locations except for the patella.Subchondral plate was thinner than C-L in the lateral plateau (-26.2%;p=0.022),groove (-19.8%;p=0.005)andpatella (-19.5%;p=0.001).Two weeks after surgery, FCD was reduced at the lateral plateau, the medial condyles, the groove, and the patella in ACLT compared to CNTRL.FCD was lower in the ACLT compared to C-L at all locations except for the lateral plateau.Interestingly, eight weeks after surgery, the FCD was increased in the medial plateau of C-L compared to CNTRL group knees.FCD loss in ACLT was progressive in the condyles, but the initial FCD loss was compensated for in the groove and patella.Also, the cartilage was thicker (50.5%;p=0.007) in the lateral plateau of ACLT knees eight weeks after surgery compared to CNTRL.Summarizing, bone loss and cartilage degeneration occurred in a tissue-and site-specific manner in post-ACLT knees and bone loss spread progressively while some compositional changes of cartilage were recovered.

Source: Bone Reports

DOI: 10.1016/j.bonr.2020.100580