FIGURE 1 from Targeting CHAF1B Enhances IFN Activity against Myeloproliferative Neoplasm Cells
Year: 2023
Type: other
Abstract: Mass spectrometry identification of cytosolic and nuclear putative binding partners of ULK1. A and B, Endogenous ULK1–protein complexes were immunoprecipitated (IP) using an anti-ULK1 antibody from cytosolic and nuclear fractions isolated from untreated (UT) or IFNα-treated [4 hours (4h)] HEL cells and analyzed by nLC/MS-MS. As negative control, IP with normal rabbit IgG was used instead of anti-ULK1 antibody and proteins bound to rabbit IgG were excluded from the analyses. A, Venn diagrams show the number of putative ULK1 binding partners identified only in untreated cells (blue), only in IFNα-treated cells (red), or both treated and untreated cells (black). CHAF1B was detected in both cell compartments by mass spectrometry. B, Venn diagram shows the overlap for the putative ULK1 binding partners identified under both untreated and IFNα-treated cells in the cytosolic and nuclear fractions. List of the putative ULK1 binding proteins found under these conditions on both cytosolic and nuclear compartments is shown. C, Gene ontology analyses of the 72 putative nuclear ULK1-interacting proteins identified under both untreated and IFNα-treated conditions are shown. Underlined and in bold are the biological events in which CHAF1B is involved.
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4378837272