FIGURE 5 from CAR T-cell Design-dependent Remodeling of the Brain Tumor Immune Microenvironment Modulates Tumor-associated Macrophages and Anti-glioma Activity
Year: 2023
Type: other
Abstract: Successful responses with 28.mζ CAR T-cells are associated with balanced proinflammatory and anti-inflammatory myeloid cell responses. Seurat clusters 0, 7, 10, 14 were reclustered into 10 Mac/MG subclusters to further define the diversity of myeloid responses after CAR T-cell treatment. A, UMAP plots of the Mac/MG subclusters visualized by treatment group. B, Summary plot of Mac/MG subcluster 2 and 7 frequencies per treatment. Volcano plots showing differentially upregulated and downregulated genes in macrophage subclusters C2 in C, C7 in D as compared with all other myeloid subclusters. E, Dot plot depicting expression of myeloid lineage markers, genes associated with protumorigenic responses, antitumorigenic, and differentiation genes. Dot size represents the percentage of cells expressing each gene and dot color represents mean expression level with a gradient of lowest expression in blue to highest expression in red. F, Dot plot depicting differentially expressed genes associated with immunosuppression, invasion, recruitment, M1-like and M2-like macrophage responses per treatment group.
Authors: Dalia Haydar, Jorge Ibañez-Vega, Jeremy Chase Crawford, Ching-Heng Chou, Clifford S. Guy, Michaela Meehl, Zhongzhen Yi, Scott Perry, Jonathan Laxton, Trevor Cunningham, Deanna Langfitt, Peter Vogel, Christopher DeRenzo, Stephen Gottschalk, Martine F. Roussel, Paul G. Thomas, Giedre Krenciute
Language: English
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4389208024