Application of laccase-inorganic nanoflowers based time-temperature integrator to real-time monitor the freshness of pasteurized milk
Year: 2024
Type: article
Abstract: A time-temperature integrator (TTI) based on laccase@Cu 3 (PO4) 2 nanoflowers (laccase@NFs) was created to monitor the freshness of pasteurized milk during storage. To address the challenges of easy inactivation, poor stability, and high use-cost of laccase in the application of TTI, laccase@NFs were synthesized by ultrasonic-assisted biomineralization. The laccase@NFs-based TTI was created through the enzymatic reaction between laccase and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid ammonium salt), and the effects of laccase@NFs addition amount on the reaction rate, discoloration lifetime and activation energy (Ea) were discussed. Furthermore, the spoilage pattern and kinetic properties of pasteurized milk were explored based on titratable acidity. The measurement of the Ea was determined as 49.98 kJ/mol, and an investigation was conducted to assess the suitability of the TTI with pasteurized milk under both constant and variable temperature conditions. This research aims to contribute valuable insights into the application of enzymatic TTI in monitoring the shelf life of pasteurized milk. • Laccase@Cu 3 (PO 4 ) 2 nanoflowers are easily synthesized by ultrasonic-assisted biomineralization. • The color of the TTI prototypes change drastically from colorless to green. • The activation energy and discoloration lifetime of the TTI are controllable. • The TTI can accurately predict the titratable acidity of pasteurized milk.
Source: Food Chemistry X
Institution: Henan University of Animal Husbandry and Economy
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $2,350
Award: G8347094281
OpenAlex ID: W4403740899