Year: 2025
Type: article
Abstract: This study is based on the extraction process of total flavonoids in traditional Chinese medicine compound, with ethanol concentration, extraction time and solid-liquid ratio as variables, three factors and three levels orthogonal experiments were conducted. We used the heat reflux extraction method to concentrate the extract. The total flavonoid content was determined using ultraviolet spectrophotometry. The optimum extraction conditions were determined using the total flavonoid content as the index. According to the experimental results, the concentration of total flavonoids in the range of 0. 0096~0. 0576 mg/ml showed a good linear relationship with absorbance. Therefore, the optimal process conditions were identified as A1B3C3: a 95% ethanol concentration; an extraction time of 120 minutes; a material-to-liquid ratio of 1:12; and a total flavonoid content of 8.273%. Orthogonal test can be used to analyze the extraction process of antibacterial components.
Authors: Zou Junyan, Tan Jingbo, Li Qiuru, Zhang Weijia, Wang Rongfan
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4413197969