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Additional file 2: Figure S2. of TriPer, an optical probe tuned to the endoplasmic reticulum tracks changes in luminal H2O2

Year: 2017

Type: article

Abstract: The role of R266 in HyPer and TriPer’s reactivity with H2O2. (A) Traces of time-dependent changes to the excitation ratio of recombinant HyPer or TriPer variants with the inactivating R266A mutation. (B) Non-reducing and reducing SDS-PAGE of recombinant TriPerR199A following incubation with increasing concentrations of H2O2 for 15 min. (C) Non-reducing gradient SDS-PAGE (pH 7.3, 4–12%) of samples as in Fig. 2g. (D) Traces of time-dependent changes to the excitation ratio of HyPer and TryPer mutant variants treated as in (B). Note that the variants lacking the ability to form C199-C208 disulfide do not change their excitation ratio upon oxidation. (PDF 93 kb)

Source: Figshare

DOI: 10.6084/m9.figshare.4792942

Authors: Melo, Eduardo, Lopes, Carlos, Gollwitzer, Peter, Lortz, Stephan, Lenzen, Sigurd, Mehmeti, Ilir, Kaminski, Clemens, Ron, David, Avezov, Edward

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W7136089368

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