Additional file 3: of Generation of lentivirus-induced dendritic cells under GMP-compliant conditions for adaptive immune reconstitution against cytomegalovirus after stem cell transplantation
Year: 2015
Type: article
Abstract: Figure S3. Analyses of the lentiviral integration sites. RG tricistronic vector (both as integrase competent and integrase defective; MOI of 5) were used to transduce monocytes for generation of SmyleDCpp65. The cells were maintained in vitro for up to 30 days. The clonal contribution of the genetically modified monocytes was monitored with a high-throughput IS analysis. (A) Number of vector copies detected by RT-q-PCR. (B) Integration pattern of ICLV and IDLV in SmyleDCpp65 in gene versus in gene ± 10 kb. (C) Integration site frequency distribution of ICLV and IDLV in SmyleDCpp65 upstream and in genes. (D) 10 Most predominant clones for ICLV and IDLV. Colored columns represent retrieval frequency as percentage of total sequences in SmyleDCpp65 at sequential time points of analyses. Larger colored bars represent higher frequencies of integration sites clustering in the proximity of that gene. Lower panel indicate ranking of the 10 most pre-dominant clones with their corresponding color code and gene ID at sequential time points of analyses. Arrows (red) indicate recurrent insertion sites observed in the analyses.
Source: Figshare
Authors: Sundarasetty, Bala Sai, Kloess, Stephan, Oberschmidt, Olaf, Naundorf, Sonja, Kuehlcke, Klaus, Daenthanasanmak, Anusara, Gerasch, Laura, Figueiredo, Constanca, Blasczyk, Rainer, Ruggiero, Eliana, Fronza, Raffaele, Schmidt, Manfred, von Kalle, Christof, Rothe, Michael, Ganser, Arnold, Koehl, Ulrike, Stripecke, Renata
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7136546850