Flavone from the Lycopersicon esculentum and their antioxidant capacity through GSH recovery effect
Year: 2021
Type: article
Abstract: The fruits of tomato (Lycopersicon esculentum) were extracted with 70% aqueous methanol (MeOH) and the concentrates were partitioned into ethyl acetate (EtOAc), n-butanol (n-BuOH), and water (H 2 O) fractions.The repeated silica gel (SiO 2 ) and octadecyl silica gel column chromatographies for the EtOAc fraction, whose activity was confirmed, led to isolation of one flavone compound.Nuclear magnetic resornance, infrarad spectroscopy, and mass spectroscopy (MS) revealed the chemical structure of the isolated compound, 5,7,3′-trihydroxy-6,4′,5′trimethoxyflavone (1).LC-MS/MS analysis determined the content level of compounds 1 in the MeOH extract to be 4.02±0.12μg/mg and in the TME-10 fraction to be 0.96±0.03μg/ mg.Through this study, the antioxidantive capacity was confirmed by demonstrating that the L. esculentum extract and their fractions showing an increase in glutathione mean and a decrease in glutathione heterogeneity uniformly raises the intracellular glutathione level.
Authors: Hyeong-Ju Jeon, Hyoung-Geun Kim
Institutions: Jangan University, Kyung Hee University
Language: English
Subfield: Biochemistry (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4200465621