Supplementary Figure S6 from LIG1 Is a Synthetic Lethal Target in BRCA1 Mutant Cancers
Year: 2025
Type: supplementary-materials
Abstract: Perturbation of LIG1 induces proportional PAR accumulation in BRCA1-mutated cells in immunofluorescence and in-cell western assay formats. A) In-cell Western blotting of PAR on treatment day 4 in MDA-MB-436 cells engineered to express a DOX-inducible LIG1 cDNA with endogenous LIG1 knockout. B) Model validation with Western blotting. C) Quantification of relative NAD+ as deduced by total NAD (NADt)/NADH ratio normalized to +DOX condition (t-test sgLIG1 +DOX vs. -DOX p < 0.0001).
Authors: Lauren Catherine M. Martires, Leanne G. Ahronian, Charlotte B. Pratt, Nikitha M. Das, Xiaobin Zhang, Douglas A. Whittington, Hongxiang Zhang, Binzhang Shen, Jon Come, Patrick McCarren, Mu-Sen Liu, Chengyin Min, Tianshu Feng, Haris Jahic, Janid A. Ali, Daniel R. Aird, Fang Li, Jannik N. Andersen, Alan Huang, William D. Mallender, Hilary E. Nicholson
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4409079255