Supplementary Figure S1 from LIG1 Is a Synthetic Lethal Target in BRCA1 Mutant Cancers
Year: 2025
Type: supplementary-materials
Abstract: Cell lines with damaging mutations in BRCA2 are dependent on LIG1. A) Analysis of DepMap data (28) comparing LIG1 dependence of cell lines with damaging mutations in BRCA2 vs. BRCA2 wild-type cell lines. ***p=0.0004 unpaired t-test. B,C) Representative image (B) and quantification (C) of colony formation assay in DLD1 wild-type (WT) and BRCA2-/- cells transfected with siRNAs targeting the LIG1 gene or a control sequence. D,E) Representative image (D) and quantification (E) of Western blot assessing LIG1 knockdown at endpoint for the colony formation assay in (B).
Authors: Lauren Catherine M. Martires, Leanne G. Ahronian, Charlotte B. Pratt, Nikitha M. Das, Xiaobin Zhang, Douglas A. Whittington, Hongxiang Zhang, Binzhang Shen, Jon Come, Patrick McCarren, Mu-Sen Liu, Chengyin Min, Tianshu Feng, Haris Jahic, Janid A. Ali, Daniel R. Aird, Fang Li, Jannik N. Andersen, Alan Huang, William D. Mallender, Hilary E. Nicholson
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4409079286