Supplementary Figure S2 from LIG1 Is a Synthetic Lethal Target in BRCA1 Mutant Cancers
Year: 2025
Type: supplementary-materials
Abstract: CRISPRi system does not reduce viability of BRCA mutant cells using an intron-targeting control guide RNA (sgITC). Representative image (A) and quantification (B) of colony formation assay (treatment day 14) in MDA-MB-436 (BRCA1 mutant) cells engineered to express a DOX-inducible CRISPRi guide RNA targeting an intron (sgITC). Knockdown was validated by Western blot (representative image in C, quantification in D). FC, fold-change.
Authors: Lauren Catherine M. Martires, Leanne G. Ahronian, Charlotte B. Pratt, Nikitha M. Das, Xiaobin Zhang, Douglas A. Whittington, Hongxiang Zhang, Binzhang Shen, Jon Come, Patrick McCarren, Mu-Sen Liu, Chengyin Min, Tianshu Feng, Haris Jahic, Janid A. Ali, Daniel R. Aird, Fang Li, Jannik N. Andersen, Alan Huang, William D. Mallender, Hilary E. Nicholson
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4409079306